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Significant effort has been invested in finding NOS selective drugs, but the results have been disappointing. This can be attributed to the fact that NOS shares the same reaction mechanism but differs in binding affinity for the cofactors and substrate of the different NOS isoforms. Although the three isoforms show high similarity in structure and chemistry, the important differences in pharmacology, enzymatic characteristics, and inducibility have led to a lack of selective drugs for the individual NOS isoforms. All three NOS isoforms, NOS1, NOS2, and NOS3, represent important targets for pharmacotherapy of cardiovascular and neurodegenerative diseases. Nitric oxide synthase (NOS) catalyzes the conversion of the amino acid L-arginine to L-citrulline and the gas nitric oxide (NO).

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